Detection of bcl-2 translocation in patients with chronic hepatitis C and its possible relation to antiviral therapy: preliminary study

ABSTRACT

It has been suggested that t[14;18] translocation of bcl-2 to the immunoglobulin heavy chain [IgH] locus may contribute to the pathogenesis of lymphoproliferative disorders [LPD] related to hepatitis C virus [HCV] infection. The present study aimed to assess the prevalence of bcl-2 translocation in Egyptian chronic HCV patients and to investigate the effect of combination antiviral therapy of interferon alpha and ribavirin on t[14;18]. Fifty five chronic HCV patients were studied for the prevalence of t[14;18]. These patients were classified into 2 groups, 33 non treated HCV patients and 22 treated HCV patients with antiviral therapy as well as control group of age and sex matched individuals. The bcl-2/IgH rearrangement was detected in peripheral blood mononuclear cells [PBMCs] by nested polymerase chain reaction. All patients have undergone HCV viral determination by real time PCR. Bcl-2/IgH translocation was detected in 21 [38.2%] of all 55 chronically infected HCV patients. Considering all patients with chronic HCV-infection, bcl-2 rearrangement was slightly more frequent in the non treated group than in those who underwent treatment with interferon plus ribavirin but the difference was not statistically significant, although treated patients showed biochemical and virologic response at the end of 6 months of antiviral therapy. In conclusion, t[14;18] in PBMCs is a frequent finding in chronic HCV infection