Expression of leukocyte adhesion molecular MAC-1 [CD11B/CD18] and L-selectin[CD62L] in patients with chronic renal failure undergoing regular hemodialysis

ABSTRACT

Mac1 and L-selectin expressed on leukocytes are critical for leukocyte adhesion to inflammed endothelium. Degranulation of polymorphnuclear leukocytes [PMN] during hemodialysis [HD] is usually assessed by measuring degranulation products. Our aim was to evaluate the effect of HD on leukocyte activation and degranulation. Fifteen normal controls and fifteen patients under chronic renal dialysis treatment were studied for Mac 1 and L-selectin expression on granulocytes by flowcytometry. PMN degranulation products; myeloperoxidase[MPO] and lactoferrin[LF] were determined in serum by ELISA method. The results of Mac 1 expression on granulocytes demonstrated a statistical significant increase in post HD compared to both pre HD [P<0.05] and control groups [P<0.05]. A statistical significant increase was also noted between Pre HD and the control group [P<0.05]. Post HD results showed a statistical significant decreased L-selectin granulocyte expression when compared to Pre HD group [P<0.05]. Post HD group showed a statistically significant increase of both MPO and LF when compared to predialysis and control group [P<0.05]. There was also a statistically significant increase in Pre HD group compared to control group in both MPO and LF levels [P<0.05].In post hemodialysis group there was a negative correlation between MAC1 and L-selectin [r=0.41]. Our finding showed that HD might influence the expression of leukocyte adhesion molecules and subsequently degranulation of PMN. Their measurement might be an indicator of PMN disturbed functions