Detection and differentiation of entamoeba histolytica and entamoeba dispar isolates in clinical samples by PCR

ABSTRACT

A total of 140 out of 180 outpatients attended MISR University for Science and Technology Hospital complained of abdominal pain, diarrhoea and/or dysentery. Stool examination showed 47 [33.6%] had Entamoeba sp., 36 [25.7%] had cysts and 11 [7.9%] had trophozoites. Of 40 asymptomatic ones, 4 [10%] had cysts. A total of 51 positive stool samples for Entamoeba sp. [40 cysts and 11 trophozoites] were tested by Ne-sted Polymerase Chain Reaction [N-PCR] and Restriction Enzyme Digestion [RED] to clarify true E. histolytica from E. dispar. The results showed that 9/51 [17.6%] had E. dispar, while 31 [60.8%] had E. histolytica and 11 [21.6%] had dual infection with both E. histolytica and E. dispar. All E. histolytica PCR proved cases were from the symptomatic group, 11 had trophozoites and 34 had cysts. Thus, the result showed the potential use of molecular tools in detection of E. histolytica and E. dispar, and is a promising tool for epidemiology, particularly to differentiate pathogenic and non pathogenic Entamoeba sp