Laboratory evaluation of modified microslide and double diffusion technique and Elisa for detection of staphylococcal enterotoxins

ABSTRACT

The modified microslide gel double diffusion test and the enzyme- linked immunosorbent assay [ELISA] were carried out on isolated strains of Staphylococci from milk and some dairy products for detection of Staphylococcal enterotoxins [A, B, C and D]. Gel double diffusion test proved that, 4 out of 36 strains of Staphylococci are enterotoxigenic and produced only enterotoxin type A. The same results were obtained also by using ESISA technique, indicating that no difference could be exist between these two methods for detection of Staphylococcal enterotoxins. Application of modified microslide gel double diffusion technique is preferable for detection of Staphylococcal enterotoxins, as it is simple, rapid, not expensive and required no complex apparatus