Modares Journal of Medical Sciences, Pathobiology. 2007; 10 (2): 11-21
em Inglês, Persa
| IMEMR
| ID: emr-84568
ABSTRACT
Recent studies suggest that intermittent and prolonged normobaric hyperoxia [HO] results in ischemic tolerance to preventing ischemia brain injury. In this research attempts were made to see the changes in excitatory amino-acid transporter 3 [EAAT3], TNF- alpha levels, and NF- kappa B activity following prolonged and intermittent NBHO preconditioning. Rats were divided into four experimental groups, each with 21 animals. The first two groups were exposed to 95% inspired HO for 4h/day for 6 consecutive days [intermittent HO; InHO] or for 24 continuous hours [prolonged HO; PrHO]. The second two groups acted as controls, and were exposed to 21% oxygen in the same chamber [nomobaric normoxia, RA; room air] continuously for six days [intermittent RA, InRA] or for 24 hours [prolonged RA; PrRA]. Each main group was subdivided to MCAO- operated [middle cerebral artery occlusion], sham-operated [without MCAO], and intact [without any surgery] subgroups. After 24 h, MCAO-operated subgroups were subjected to 60min of right MCAO. After 24h reperfusion, neurologic deficit score [NDS] were assessed in MCAO-operated subgroups. Immediately and 48 h after pretreatment, blood sampling for assessment of serum TNF- alpha levels were performed. Then, the effect of InHO and PrHO on serum TNF - alpha levels, NF - kappa B activity and EAAT3 expression were measured. Reconditioning with InHO and PrHO decreased NDS and upregulate EAAT3 and increase serum TNF- alpha level and NF- kappa B activity significantly. Although further studies are needed to clarify the mechanisms of ischemic tolerance, InHO and PrHo seems to partly exert their effects via increase in serum TNF- alpha levels, NF- kappa B activity and upregulation of glutamate transporters
Buscar no Google
Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Ratos
/
Isquemia Encefálica
/
NF-kappa B
/
Fator de Necrose Tumoral alfa
/
Sistema X-AG de Transporte de Aminoácidos
Limite:
Animais
Idioma:
Inglês
/
Persa
Revista:
Modares J. Med. Sci., Pathobiol.
Ano de publicação:
2007
Similares
MEDLINE
...
LILACS
LIS