Capillary network formation by endothelial cells differentiated from human bone marrow mesenchymal stem cells
IJB-Iranian Journal of Biotechnology. 2008; 6 (1): 29-35
em Inglês
| IMEMR
| ID: emr-86728
ABSTRACT
Human bone marrow derived mesenchymal stem cells [HBMSCs] have the potential to differentiate into cells such as adipocyte, osteocyte, hepatocyte and endothelial cells. In this study, the differentiation of hBMSCs into endothelial like-cells was induced in presence of vascular endothelial growth factor [VEGF] and insulin-like growth factor [IGF-1]. The differentiated endothelial cells were examined for their ability to express VEGF receptor-2 [VEGFR2] and von willebrand factor [vWF]. Then the cells were adopted to grow and develop capillary network in a semisolid gel matrix in vitro. The capillary network formation in a well of 24-well plate was found to be 85% in presence of VEGF [50ng/ml] and IGF-1 [20ng/ml] of the culture media. These data may suggest that the expression of endothelial markers in endothelial like-cells derived from hBMSCs is associated with their ability to form capillaries
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Células da Medula Óssea
/
Biomarcadores
/
Diferenciação Celular
/
Receptor 2 de Fatores de Crescimento do Endotélio Vascular
/
Células Endoteliais
/
Células-Tronco Mesenquimais
Limite:
Humanos
Idioma:
Inglês
Revista:
Iran. J. Biotechnol.
Ano de publicação:
2008
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