Genetic variations of avian Pasteurella multocida as demonstrated by 16S-23S rRNA gene sequences comparison
Iranian Journal of Veterinary Research. 2008; 9 (3): 240-244
em Inglês
| IMEMR
| ID: emr-87309
ABSTRACT
Pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. A polymerase chain reaction [PCR] assay was developed using primers derived from conserved part of 16S-23S rRNA gene. The PCR amplified a fragment size of 0.7 kb using DNA from nine avian P. multocida isolates. Sequence alignment of the 16S-23S rRNA genes [ITS] revealed a considerable heterogenicity among the isolates. The percentage of similarity varied from 83.3 to 100% among the isolates. An interesting finding from this study was the presence of an inserted sequence [seven nucleotides] in the 16S-23S rRNA region in 55% of the isolates. According to phylogenic analysis based on ITS sequence alignment, the P. multocida isolates classified into 2 distinct clusters. The virulence of isolates in cluster II were higher than those in cluster I. Ribotyping of P. multocida by using 16S-23S rRNA gene PCR sequencing could be used as a marker in epidemiologic studies
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Fenótipo
/
Variação Genética
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de RNA
/
Genes de RNAr
/
Ribotipagem
Limite:
Animais
Idioma:
Inglês
Revista:
Iran. J. Vet. Res.
Ano de publicação:
2008
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