efficacy of turmeric to alleviate chlorinated solvents-induced oxidative status on rats

ABSTRACT

Purpose is to examine the possible influence of turmeric as natural antioxidant on 1,2-dichloroethane [1,2DCE] induced oxidative status in rats. Thirty five adult male albino rats Sprague-Dawley strain were divided into five groups [7rats each]. Group A served as negative control fed basal diet. Group B [positive control] fed standard diet and 1,2DCE added [313mg / 100 g diet] for 4 weeks. Group C fed standard diet + 1,2DCE + curcumin 0.5% for 4 weeks as a protective group. Group D fed standard diet + curcumin 0.5% for 4 weeks after that added 1,2 DCE to 4 weeks as a preventive group. Group E fed standard diet + 1,2DCE for 4 weeks after that added turmeric 0.5% for 4 weeks as a curative group. Levels of Glutathione [GSH], Superoxide dismutase [SOD] and Malondialdehyde [MDA] were determined in homogenate kidneys, brain, and lungs of rats. RNA and DNA were extracted from brain homogenate. Determination of serum total protein, urea, uric acid and creatinine. The results showed that both of GSH, SOD, RNA and DNA increased in rats' tissue for treated groups with turmeric but MDA is decreased versus positive groups. The analysis of serum explains alleviating the adverse effect of chlorinated solvents on rats that fed turmeric. kidney, lung and brain, these organs representing important target organs of chlorinated solvents toxicity and the turmeric as a natural antioxidants alleviated effect of these pollutant