Dose related effect of systemic antibiotics in prevention of postoperative intra-abdominal adhesion formation in experimental animals

ABSTRACT

Sixty hamsters weighing 100-120 g were randomly assigned to 2 equal groups. GI was injected intramuscularly with saline, half an hour preoperatively as control, and GII was injected with 50 mg/kg Cefepime HC1 and 7.5 mg/kg Metronidazole. After a midline lapparo-tomy, abdominal adhesions were induced in GI and GII Post-operration, animals in GI was divided according to the numbers of intramuscular saline injections into 2 subgroups. GIa[15] in which animals were injected every 12 hours for 2 doses and GIb [15] where animals were injected every 12 hours for 5 days. Similarly, the antibiotic group was subdivided into GIIa [15] and GIIb [15]. On the 14[th] day, the hamsters were sacrificed and the adhesion score was determined. The 5 day antibiotics course revealed significant reduction in incidence [P < 0.01], extent [P < 0.001] and severity [P < 0.01] of the postoperative peritoneal adhesions, while the short course failed