Genotyping of toxoplasma gondii strains in pregnant women with toxoplasmosis in Gezira state, central Sudan

ABSTRACT

To perform genotyping of Toxoplasma gondii among pregnant women with toxoplasmosis in Central Sudan. The study group was presented by 94 pregnant women who had abortion between the second and fourth months of gestation, whereas the control group consisted of 64 full term normally delivered women. The study was conducted at Wad Medani Teaching hospital, Gezira State, Central Sudan during the period of March 2002 to May 2004. PCR test on aborted and placental tissue revealed positivity rate of 19.1% and 22.3%, in study and control groups respectively, with no significant difference between them [P=0.55]. This compared to a seroprevalence rate of 35.1% and 39.4% in study and control groups respectively. Genotyping form clinical samples using PCR/RELP revealed Toxoplasma gondii type II in 89% and 95% of the study and control group respectively, whereas, 11% and 5% were infected with type III. The most prevalent strain of T. gondii among the pregnant women was Type II followed by type III but type I was completely absent