[Molecular diagnosis of B cell Non-Hodgkin Lymphoma by evaluation of immunoglobulin heavy chain gene rearrangement]
Scientific Journal of Iranian Blood Transfusion Organization Research Center [The]. 2008; 5 (2): 157-166
em Persa
| IMEMR
| ID: emr-90312
ABSTRACT
Rearrangement of V, D, and J segments of immunoglobulin heavy chain gene with inserted or deleted nucleotides within rearranged segments makes unique hypervariable regions [CDR-3]. These regions can be used for evaluation of B cell clonality for the purpose of molecular diagnosis of Non-Hodgkin Lymphoma [NHL] and for confirmatory diagnosis in suspicious cases. In this study, samples of 42 patients were collected from Taleghani, Baqhiyatalah, and Aliasghar hospitals; out of this number, there were 22 patients with diagnosis of B cell NHL, 10 with reactive hyperplasia, and 10 with malignant lymphoma. After DNA extraction from formalin fixed paraffin embedded tissues, PCR was done using consensus primers for amplification of CDR-3 region. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis and silver stain. Results Clonal patterns in group 1 [B cell NHL], 2 [reactive and follicular hyperplasia], and 3 [morphological diagnosis without immunohistochemistry] were observed in 77.2%, 0%, and 70% of patients, respectively. Our findings are compatible with other international studies with minor differences. The diagnosis of B-cell lymphoid malignancy can frequently be substantiated by detecting clonal immunoglobulin heavy chain [IGH] gene rearrangement
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IMEMR (Mediterrâneo Oriental)
Assunto principal:
Linfoma não Hodgkin
/
Rearranjo Gênico de Cadeia Pesada de Linfócito B
/
Reação em Cadeia da Polimerase
/
Cadeias Pesadas de Imunoglobulinas
/
Técnicas de Diagnóstico Molecular
Limite:
Humanos
Idioma:
Persa
Revista:
Sci. J. Iran. Blood Transfus. Organ. Res. Cent.
Ano de publicação:
2008
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