Genetic fingerprints of some bacillus spp. isolates gathered from different locations at Saudi Arabia

ABSTRACT

Twelve Bacillus spp. isolates gathered from different locations at Saudi Arabia were fingerprinted by random amplified polymorphic DNA [RAPD] markers. The results revealed high levels of polymorphism among the studied isolates. Of many 10-mer random primers screened, five gave distinct and reproducible polymorphic DNA amplification patterns. The primers are A3, B6, B10, B11 and C12. The number of the generated bands was primer and genotype dependent. A total of 36 amplified polymorphic DNA bands were generated across the studied isolates with 9 bands being unique. All primers gave a number of unique bands that used for discrimination of some isolates. No primer was able to generate a unique banding pattern for all the studied isolates. However, combinations of all data derived from the RAPD profiles generated by the five utilized primers produced a unique fingerprint for each isolate. Similarity coefficients among the studied isolates were estimated after pooling all the gained data. The levels of similarity between the studied isolates showed low values. It reached the highest value of 0.938 between the two isolates N2 and M2, while the minimum value was 0.118 recorded between the two isolates M1 and W1. The generated dendrogram clustered the three isolates E1, N2 and M2 together and located W1 in a separate cluster