Lipoprotein lbpase mRNA expression in different tissues of farm animals
EJB-Egyptian Journal of Biochemistry and Molecular Biology [The]. 2009; 27 (1): 107-116
em Inglês
| IMEMR
| ID: emr-91050
ABSTRACT
Lipoprotein lipase [LPL] controls triacylglycerol partitioning between adipose tissues and muscles, so it is important enzyme for fattening of animals.The present work was planned to clarify the use of polymerase chain reaction [PCR] for detection of LPL mRNA expression in different tissues representing internal organs of male Friesian cows. The obtained results revealed that, LPL mRNA was expressed in all tested tissues, and the highest expression was encountered in testis [1.03]. The expression in heart, liver, kidney, adrenal gland, spleen were 0.92, 0.77, 0.76, 0.73, and 0.70 respectively.The lowest expression was found in adipose tissue [0.64] It could be concluded from the present study that, expression of LPL mRNA differs according to the type of tissue. The highest expression was found in testis which may confirm the role of LPL in the process of spermatogenesis
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Testículo
/
Triglicerídeos
/
RNA Mensageiro
/
Bovinos
/
Tecido Adiposo
/
Reação em Cadeia da Polimerase
/
Eletroforese em Gel de Ágar
/
Coração
/
Lipase Lipoproteica
/
Fígado
Limite:
Animais
Idioma:
Inglês
Revista:
Egypt. J. Biochem. Mol. Biol.
Ano de publicação:
2009
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