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Determination of HBV genotypes among HBs Ag positive blood donors in Tehran, Iran using PCR-RFLP
Iranian Journal of Public Health. 2009; 38 (1): 41-47
em Inglês | IMEMR | ID: emr-91465
ABSTRACT
Hepatitis B virus [HBV] is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a comparison of complete genomic sequences, HBV has been classified into eight genotypes A-H which show a geographical distribution. Some genotypes are associated with different clinical outcomes. Identification of HBV genotypes is important to begin and follow up the treatment. In this cross-sectional study, the serum samples of blood donors were collected from Tehran Blood Transfusion Centers in period during "2005-2006". Sera of 55 blood donors who were positive for hepatitis B surface antigen were selected. DNA was extracted using commercial kit and the S gene sequence was amplified by nested-PCR. PCR products were then analyzed for restriction enzymes that would be genotype specific. Genotype D was found the only type in all HBV DNA positive serum samples, in Tehran. Genotype D is dominant among Tehran's blood donors, which is consistent with Iran and the Middle East dominant genotype
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Índice: IMEMR (Mediterrâneo Oriental) Assunto principal: Doadores de Sangue / Polimorfismo de Fragmento de Restrição / Reação em Cadeia da Polimerase / Estudos Transversais / Genótipo / Antígenos de Superfície da Hepatite B Tipo de estudo: Estudo de prevalência Limite: Feminino / Humanos / Masculino Idioma: Inglês Revista: Iran. J. Public Health Ano de publicação: 2009

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Índice: IMEMR (Mediterrâneo Oriental) Assunto principal: Doadores de Sangue / Polimorfismo de Fragmento de Restrição / Reação em Cadeia da Polimerase / Estudos Transversais / Genótipo / Antígenos de Superfície da Hepatite B Tipo de estudo: Estudo de prevalência Limite: Feminino / Humanos / Masculino Idioma: Inglês Revista: Iran. J. Public Health Ano de publicação: 2009