Localization of Epidermal-Type Fatty Acid Binding Protein in Degeneration and Regeneration of Sciatic Nerve after Crush Injury in Mouse

ABSTRACT

The regeneration of axon and myelin sheet after crush injury of peripheral nerves involves interaction of several types of cells. including Schwann cells, monocyte, niacrophage and fibrohiast. Among them, haeniatogenous macrophages invading into the peripheral nervous system play a major role in myelin uptake during Wallerian degeneration. In this study 35 C57IBL6male mice 10 weeks old were used and classified in 7 groups of 5 mice. The localization of epidermal-type fatty acid binding protein [E-FABF] in the mature mouse sciatic nerve after crush injury was examined by immuno-light microscopy. Numerous macrophages immunopositive for both anti-E-FABP and F4/80 a macrophage marker, were found in degeneration process of sciatic nerve. Macrophages contained phagosomes of various sizes and they were regarded as those actively involved in the phagocytosis of sciatic nerve debrise. Conclusion: The present dietection of E-FABP immunopositivity selectively in invading macrophages suggests possible involvement of E-FABP and/or its fatty acids ligand in the process of peripheral nerve regeneration