[Study on interferon gamma gene polymorphism in shaul sheep]
Journal of Veterinary Research. 2009; 64 (3): 199-204
em Persa
| IMEMR
| ID: emr-93795
ABSTRACT
Interferon gamma [IFN-gamma] is a cytokine produced by activated T lymphocytes and natural killer cells. It plays an important role in innate and adaptive immunity. IFN-g gene is polymorphic and its haplotype has been associated with resistance to nematode infestations which are the most important parasites of domestic sheep worldwide. To evaluate IFN-g polymorphism in Iranian Shaul sheep population, 136 blood samples were collected. Genomic DNA were extracted and amplified by PCR using specific primers for exon 3 of IFN-g gene. After gene amplification, SSCP and Heteroduplex mobility analysis carried out on polyacrylamid gel. Five unique SSCP patterns were detected in this population which 3 of them represent 3 alleles. Although heteroduplex mobility analysis demonstrated that all samples were homozygous, SSCP could reveal heterozygosity at frequency of 40. 4% in the studied population. Data obtained from the present study have revealed that IFN-g gene was a highly polymorphic in Iranian Shaul ecotype. This will be useful to study host-pathogen interaction and to evaluate association between resistance or susceptibility to disease with IFN-g in this ecotype
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Polimorfismo Genético
/
Doenças dos Ovinos
/
Reação em Cadeia da Polimerase
/
Polimorfismo Conformacional de Fita Simples
/
Análise Heteroduplex
Limite:
Animais
Idioma:
Persa
Revista:
J. Vet. Res.
Ano de publicação:
2009
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