Effect of fetal calf serum on cellular proliferation of mouse Y1 adrenocortical cells in vitro

ABSTRACT

Fetal calf serum [FCS] is a supplement used in cell culture media for successful culturing. The present study was design to observe the effect of FCS on cellular proliferation. Effect of different concentrations [0, 1, 5, 10 and 20%v/v] of FCS on cellular proliferation was determined by the uptake of crystal violet, MTT assay. Total cellular protein was also measured colorimetrically to observe the effect of FCS on growth of mouse Y1 adrenocortical cells. The results showed a gradual increase in proliferation of Y1 cells by increasing concentrations of FCS in Dulbecco's modification of Eagle's medium [DMEM]. Highest proliferation rate of Y1 adrenocortical cells was achieved with cultured cells after 6 days in DMEM medium containing 10 to 20% FCS. The study suggested that supplementation of 20% FCS increase cell proliferation and acts as a growth factor results in cell division and DNA synthesis: