Development of Egyptian barley with HMW-GS Dy10 gene of wheat
Arab Journal of Biotechnology. 2008; 11 (2): 159-168
em Inglês
| IMEMR
| ID: emr-94498
ABSTRACT
Transgenic barley plants with high-molecular-weight glutenin subunit [HMW-GS] gene from wheat were successfully generated. The HMW-GS Dy10 gene, known to be essential in bread industry, was introduced into the Egyptian barley cv. Giza 123 by biolistic bombardment. The transgenic plants, regenerated from immature embryo-derived callus cultures, were normal and fertile. Stable integration of Dy10 transgene was confirmed by molecular analysis and its expression was studied by protein analysis. Dy10 gene was co-transformed into barley with the plasmid pAHC25 harboring the bar gene for selection and the gus reporter gene. Production of Egyptian barley with wheat HMW-GS genes could be used to develop barley flour with the unique properties of wheat flour for use in bread industry
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Hordeum
/
Plantas Geneticamente Modificadas
Idioma:
Inglês
Revista:
Arab J. Biotechnol.
Ano de publicação:
2008
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