Nutritional requirements for gelatinase biosynthesis by Streptomyces spheroides

ABSTRACT

effect of some nutritional factors on gelatinase biosyn-thesis by Streptomyces spheroides was studied. The experimental organism was cultivated at static conditions at 30° and pH 7.0 for 8 days. Starch-nitrate medium was used throughout this work. 0.3% gelatin gave the maximum gelatinase activity among the tested organic and inorganic nitrogen sourced, whereas 2.5% glucose and potassium dibasic phosphate [0.15%] were the most suitable carbon and phosphorus sources, respectively; for gelatinase biosynthesis. The effect of different calcium carbonate concentrations was also tested, 0.1% was the best concentration for gelatinase biosynthesis by Streptomyces spheroides