Delta 1-Dehydrogenation of cortisol with bacteria III- Some biochemical aspects of delta 1 dehydrogenation of cortisol with Corynebacterium equi

ABSTRACT

The enzymic transformation of cortisol with Corynebacterium equi was examined in the presence of some biological bases, redox agents, enzyme inhibitors and steroid inducers. Supplementation of the reaction mixture with adenine and adenosine retarded the formation of prednisolone, while uracil and xanthine led to the formation of high yields of prednisolone. The enzymic delta 1-dehydrogenation reaction of cortisol was accelerated in the presence of oxidized glutathione. The bioconversion of cortisol to predniso[lone, was also activated in the presence of azide and cyanide. With iodoacetate, the delta 1-Dehydrogenation reaction was promoted. The delta 1-Dehydrogenation reaction of cortisol was considerably induced when the cells, were pretreated with delta 1 Dehydroprogesterone, and delta 1-dehydrotestosterone