Expression of human vitamin D3 receptor in Ecoli

ABSTRACT

The ability to produce large quantities of receptor is important for understanding certain fundaments mechanisms of action of the vitamin D3 receptor [VDR]. Conventional receptor isolation techniques can provid only small quantities of purified receptor. The method presented in this study utilizes a ubiquitin fusion technology and a low temperature [< 30 §C] nalidixic acid induction of the lambda PL promoter. Using this nove method, we have overexpressed the biologically active form of human vitamin D3 receptor [hVDR] in E. coli