Androgen binding site in macrophages
IJMS-Iranian Journal of Medical Sciences. 1997; 22 (1-2): 32-37
em Inglês
| IMEMR
| ID: emr-96055
ABSTRACT
The presence of androgen binding sites [five alpha dihydrotestosterone [5%-DHT]] on NZB/BALBc mouse peritoneal macrophages [M phi] was identified. Cells were incubated with different concentrations of [3H]-5 alpha-DHT in the presence or absence of a 100 fold excess of unlabelled 5 alpha-DHT to correct for nonspecific binding. Labelled cells were separated from unbound steroid by immunomagnetic beads coated with rat anti-mouse M phi antibody [rat anti-mouse F4/80 Ag]. The binding identified in the mouse M phi was highly selective towards androgenic compounds. Little competition was seen from 17 beta oestradiol [17beta E2] or the synthetic glucocorticoid triamcinolone acetonide. Binding data derived from Scatchard analysis showed the presence of a high affinity androgen binding species. The dissociation constant [Kd] value for the receptor was calculated to be 4.7x10[-9]M. The number of receptors per cell was 2950
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Hormônios Esteroides Gonadais
/
Di-Hidrotestosterona
/
Sítios de Ligação
/
Receptores Androgênicos
/
Macrófagos Peritoneais
/
Sistema Imunitário
/
Androgênios
/
Camundongos
Limite:
Animais
Idioma:
Inglês
Revista:
Iran. J. Med. Sci.
Ano de publicação:
1997
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