Evaluation of Juglans regia pericarp on antifungal susceptibility with broth dilution method

ABSTRACT

Antifungal property of Juglans regia has not been proved yet, therefore, the present study we designed to examine the effect of Juglans regia pericarp on the growth of different fungi, particularly dermatophytes. The extract has been prepared by methanol solution using percolation method according to NCCLS protocol. Then, the fractions have been examined on fungi inoculums. For MBC estimation, it has been cultured on PDA medium. Juglans regia extract completely prevented growth of Epidermophyton floccosum and Trichophyton mentagrophytes in fraction of 120 mg/ml, Microsporum canis in fraction of 450 mg/ml, and Candida albicans in fraction of 337 mg/ml, meanwhile, it reduced colony growth of Aspergillus niger compared with the control. We have demonstrated that the growth of three dermatophyes and Candida albicans was completely inhibited by Juglans regia pericarp extract