Bacterial adherence: the role of serum and wound fluid

ABSTRACT

Bacteria are known to initiate wound infections and have been found associated with wound infections. The purpose of this study was to investigate the bacterial adherence potential [BAP] of isolates commonly isolated from wounds in the presence of wound agents. The bacteria used were Pseudomonas aeruginosa, Proteus mirabilis, Escherichia coli and Staphylococcus aureus which were isolated from infected wounds and assayed for their adherence ability using wound fluid and serum. The BAP were achieved by exposing the pathogens to freshly excised wounds. The adhered bacteria were then eluded and quantified using log [CFU]/cm[2] on Mueller Hinton Agar per cm[2] of tissue. The results indicated that wound fluid and serum have a remarkable bacterial adherence potential [BAP] when exposed to freshly injured wounds as when compared to distilled water and no agent