In vitro differentiation of Wuchereria bancrofti (Filariidae)
Braz. j. med. biol. res
;
24(10): 1011-6, 1991. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-102081
ABSTRACT
Wuchereria bancrofti microfilariae were isolated from blood of infected individuals and cultured in vitro under several conditions. RPMI 1640 and TC-199 media supplemented with fetal or human serum were able to support the microfilariae for periods up to 35 days at 37-C (viability > 85%). In contrast, in minimal essential medium the microfilariae did not survive for more than 48h. In culture kept at 28-C, where viability was much lower (approximately 10% on day 15), microfilariae differentiated into type IV larvae ("sausage form). The in vitro maintainance of microfilarial larval forms is particularly important in the case of W. bancrofti due to the absence of an experimental model for the disease
Buscar no Google
Índice:
LILACS (Américas)
Assunto principal:
Wuchereria bancrofti
/
Técnicas In Vitro
/
Diferenciação Celular
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1991
Tipo de documento:
Artigo
/
Documento de projeto
Similares
MEDLINE
...
LILACS
LIS