Near-ultraviolet illumination inhibits the liquid holding recovery in Escherichia coli B

ABSTRACT

Liquid holing recovery (LHR) consist of an increase in the survival of UV-irradiated cells when they ar held under non-nutrient conditions before palting. In this study we investigated in E. coli B cells the effect of the growth inhibition induced by near UV (365 nm) illumination (growth delay, GD) before irradiation with UV-254 nm on the amplitude of LHR and the induction of an SOS function (filamentation) during the liquid holding period. Our results demonstrated that pre-illumination with near-UV inhibitis LHR and the induction of filamentation when the cells are incubated in nutrient medium. Moreover, this inhibition is due to GD, an effect caused by a photoproduct in the E. coli t-RNA, the 8-13 link