Comparison of indirect immunofluorescencee with agar gel immunodiffusion for the diagnosis of bluetongue virus infection
Braz. j. med. biol. res
;
25(5): 503-8, 1992. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-109057
ABSTRACT
Sera from 190 cows and from 72 sheep were examined to compare the results obtained with the agar gel immundiffusion (AGIP) and indirect immunofluorescence (IIF) tests for the diagnosis of bluetongue (BT) disease. In the AGIP test, 96 of 190 (50.5%) cattle serum samples and 38 of 72 (52.7%) sheep serum samples were positive, for a total of 134 out of 262 (51.1%) sera. In the IIF test, 98 of 190 (51.6%) cattle serum samples and 39 of 72 (54.2%) sheep serum samples were positive, for a total of 137 out of 262 (52.3%) sera. The fluorescence of the IIF test presented a granular cytoplasmic aspect, which in some cells was observed only on the cell membranes. Statistical analysis of the data showed close agreement between the two techniques, regardless of the kind of sera examined. The IIF test showed high sensitivity (93.8% and 92.1%), specificity (91.4% and 88.2%) and positive (91.8% and 89.7%) and negative (93.48% and 90.9%) predictive values for cattle serum and sheep serum, respectively. The results obtained with obtained with IIF were comparable to those obtained with the AGIP test, indicating that both techniques can be used routinely in epidemiologic studies of BT. However, the IIF offers the additional advantages that it can be used for antibody quantification and for the detection of viral antigens in BT-infected cell lines
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Índice:
LILACS (Américas)
Assunto principal:
Ovinos
/
Imunofluorescência
/
Imunodifusão
/
Bluetongue
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1992
Tipo de documento:
Artigo
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