Cloning of a B10 DNA puff sequence developmentally amplified and expressed in the salivary gland of Bradysia hygida
Braz. j. med. biol. res
;
25(8): 777-80, 1992. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-113568
ABSTRACT
A recombinant clone carrying a 2-kb fragment was isolated from a mini-library of the B10 DNA puff of Bradysia hygida. This fragment was amplified in the salivary gland during the period of DNA puff formation. Amplification started when DNA puff anlage was formed and continued to increase, reaching a maximum of abouth 10-fold 28 h later. Northern blot hybridization experiments showed that this 2-kb fragment was complementary to two RNA species of about 1.3 kb and 1.1 kb, which are developmentally regulated in the salivary gland. Maximum amounts of these messages were present when the B10 puff is fully expanded
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Índice:
LILACS (Américas)
Assunto principal:
Glândulas Salivares
/
Transcrição Gênica
/
DNA
/
Amplificação de Genes
/
Clonagem Molecular
/
Drosophila
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1992
Tipo de documento:
Artigo
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