Detection of cytomegalovirus in urine of HIV-infected patients by DNA-DNA hybridization comparison with virus isolation, immunofluorescence and immunoperoxidase
Mem. Inst. Oswaldo Cruz
;
87(1): 99-102, jan.-mar. 1992. tab
Artigo
em Inglês
| LILACS
| ID: lil-116289
RESUMO
Immunofluorescence and immunoperoxidase test directed against early viral antigens, and DNA-DNA hybridization were compared with viral isolation for their abilities to detect Cytomegalovirus (CVM) in the urine of 89 HIV infected patients. From the 100 urine samples collected, 70 were found positive by at least one method. Considering viral isolation as the "gold standard" technique, immunofluorescence and immunoperoxidase had a sensitivity of 92.3% and88% respectively, with a specificity in both cases of 95%. DNA-DNA hybridization showed a sensitivity of 90% but with lower (60%) specificity. All of the three assays were effective in detecting CVM from urine and the technical advantage of each is discussed.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Adjuvantes Imunológicos
/
Imunofluorescência
/
Citomegalovirus
/
Hibridização Genética
/
Anticorpos Monoclonais
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
1992
Tipo de documento:
Artigo
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