Simplified method for the analysis of cellular karyotype and phenotype in leukemias
Rev. paul. med
;
110(3): 97-101, May-Jun. 1992. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-134383
ABSTRACT
The objective of this study was to develop a simplified method for the simultaneous analysis of cellular karyotype and phenotype which would permit the identification of cell origin. We studied 6 patients with AML, 3 with CML (one of which was in blastic transformation) and one ALL. We used a method in which the suspension of bone marrow cells was incubated in TC 199 medium with colchicine and with hypotonic solution formed from glycerol, NaCl, KCl, CaCl2, MgCl2 and sucrose. The slides were prepared from this cell suspension by cytospin and stained for peroxidase, PAS, esterases and iron. The karyotype was studied by direct method and culture. It was possible to relate the cytogenetic marker with cytochemistry characteristics in the same cell in 3 cases, showing the feasibility of cytochemistry techniques in cytogenetical preparations. The best preparations were found through peroxidase. The presence of iron granules allowed identification of erythroblastic lineage in the combined staining. Mitosis with a marker chromosome of leukemic clone in an AML cell with negative peroxidase probably showed a proliferation of more primitive precursor not sufficiently differentiated to show markers
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Índice:
LILACS (Américas)
Assunto principal:
Leucemia Mielogênica Crônica BCR-ABL Positiva
/
Crise Blástica
/
Leucemia Mieloide
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Rev. paul. med
Assunto da revista:
Medicina
Ano de publicação:
1992
Tipo de documento:
Artigo
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