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Biochemical analysis of the methylic antigen of Paracoccidioides brasiliensis
Rev. Inst. Med. Trop. Säo Paulo ; 34(6): 511-6, nov.-dez. 1992. tab
Artigo em Inglês | LILACS | ID: lil-134554
ABSTRACT
Yeast forms of five strains of Paracoccidioides brasiliensis (SN, 2, 18, 192 and JT-1) were cultured in a synthetic medium for obtaining methylic antigens. These antigens were lyophilized and studied for each strain, to determine their partial biochemical composition, through measurements of total lipid, protein and carbohydrate contents. Lipids of methylic antigens were purified and analysed for sterols, phospholipids, glycolipids, lipoproteins, and partial characterization of sterols. Significant differences were found among antigenic preparations derived from distinct P. brasiliensis strains, in relation to the quantitative determinations. On the other hand, sterol analysis revealed the presence of ergosterol, lanosterol and squalene in all samples. The diversity verified in the biochemical characteristics of antigens derived from different P. brasiliensis strains, confirm the need of using a pool of fungal samples in order to produce antigen preparations for serological procedures without hampering their sensitivity
Assuntos
Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Paracoccidioides / Antígenos de Fungos País/Região como assunto: América do Sul / Brasil Idioma: Inglês Revista: Rev. Inst. Med. Trop. Säo Paulo Assunto da revista: Medicina Tropical Ano de publicação: 1992 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Paracoccidioides / Antígenos de Fungos País/Região como assunto: América do Sul / Brasil Idioma: Inglês Revista: Rev. Inst. Med. Trop. Säo Paulo Assunto da revista: Medicina Tropical Ano de publicação: 1992 Tipo de documento: Artigo