The role of glycolipid C in the GPI biosynthetic pathway in Trypanosoma brucei bloodstream forms
Braz. j. med. biol. res
;
27(2): 121-6, Feb. 1994. ilus
Artigo
em Inglês
| LILACS
| ID: lil-138274
ABSTRACT
The glycosylphosphatidylinositol (GPI) biosynthetic pathway in Trypanosoma brucei bloodstream forms includes the formation of glycolipid C. This molecule is the inositol-acylated form of the GPI anchor precursor, glycolipid A. There is no evidence for the transfer of glycolipid C to protein in vivo and the role of glycolipid C is unclear. In this paper we show that glycolipid C is not synthesised in the presence of phenylmethylsulphonyl fluoride (PMSF) and that glycolipid C is not an obligatory intermediate on the pathway to the formation of glycolipid A. Using pulse-chase experiments we show that glycolipid A and glycolipid C are in a dynamic equilibrium and we suggest that only the forward reaction (glycolipid A conversion to glycolipid C) is inhibited by PMSF
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Índice:
LILACS (Américas)
Assunto principal:
Fosfatidilinositóis
/
Trypanosoma brucei brucei
/
Glicolipídeos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1994
Tipo de documento:
Artigo
/
Congresso e conferência
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