A trap system for the isolation of amino acid sequences inducing GPI anchor addition
Braz. j. med. biol. res
;
27(2): 189-93, Feb. 1994. ilus
Artigo
em Inglês
| LILACS
| ID: lil-138284
ABSTRACT
We designed a trap system to isolate different amino acid sequences which could target proteins to the cell surface via GPI anchor transfer. This selection procedure is based on the insertion of various sequences which regenerate a functional GPI anchor signal sequence and therefore provoke re-expression at the surface of a reporter molecule. Using this trap for cell surface targeting sequences, we could show the importance of the defined elements essential for GPI anchor addition. Such a system could be used for an exhaustive analysis of the carboxyl terminus structural requeriments for GPI membrane anchoring
Buscar no Google
Índice:
LILACS (Américas)
Assunto principal:
Fosfatidilinositóis
/
Glicolipídeos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1994
Tipo de documento:
Artigo
/
Congresso e conferência
Similares
MEDLINE
...
LILACS
LIS