Decrease in the ratio of high to low affinity isozymes of (Na+ +K+)-ATPase during the development of rat cardiac ventricles
Braz. j. med. biol. res
;
28(3): 363-7, Mar. 1995. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-154705
ABSTRACT
The ratio of (Na++K+)-ATPase (EC 3.6.1.3.) isoforms with high and low affinity for cardiac glycosides was studied in heart preparations from neonatal, 3-month and 6-month old Wistar rats. Biphasic ouabain inhibition curves of (Na++K+)-ATPase activity indicated that the relative contribution of the high-affinity process decreased from 34 percent at 9 days to 23 percent at 3 months and to 10 percent at 6 months. Scatchard plots for [3H]ouabain binding were curvillinear and indicated that the relative contribution of the high-affinity sites (Kd = 0.1-0.25 µM) decreased by about one-half between 3 months (19-24 percent, N = 2) and 6 months (9-11 percent, N = 2)
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Índice:
LILACS (Américas)
Assunto principal:
Ouabaína
/
ATPase Trocadora de Sódio-Potássio
/
Ventrículos do Coração
/
Isoenzimas
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1995
Tipo de documento:
Artigo
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