Participation of TNF-Ó and IL-1 in the pathogenesis of cyclophosphamide-induced hemorrhagic cystitis
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;28(10): 1103-8, Oct. 1995. graf
Article
em En
| LILACS
| ID: lil-161002
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BR1.1
ABSTRACT
The involvement of cytokines TNF-Ó and IL-1 has been investigated in a model of cyclophosphamide (CYP) - induced hemorrhagic cystitis. Male Swiss mice (25-30 g) received CYP in a single ip dose of 100, 200 or 400 mg/kg and were sacrificed 6, 12, 24, 48 and 72 h later. Cystitis was evaluated by determining the changes in bladder wet weight (BW) and plasma protein extravasation (PPE, measured by the Evans blue leakage technique). CYP treatment induced a marked increase in BW and in PPE, which was significant within 6 h and reached maximal values within 12 h (BW, 118 percent, P<0.05; N = 11; and PPE, 824 percent, P<0.05; N = 11), continuing to be significant until 48 h. Pretreatment of animals with whole anti-TNF-Ó serum (25 or 50 µl diluted in 500 µl 0.9 percent saline, ip, 30 min earlier caused a significant reduction in the CYP-induced BW increase in 6-h and 12-h cystitis (82 percent and 91 percent, respectively, P<0.05; N = 6) and...
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Índice:
LILACS
Assunto principal:
Interleucina-1
/
Fator de Necrose Tumoral alfa
/
Ciclofosfamida
/
Cistite
Tipo de estudo:
Etiology_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1995
Tipo de documento:
Article
/
Congress and conference