Isolation and axenization of two entamoeba hitolytica strains / Aislamiento y axenización de dos capas de entamoeba histolytica

ABSTRACT

We describe the isolation and axenization of two E. histolyca strains, obtained from the stools of two patienst with the clinical diagnosis of dysentery. We used Pavlova's medium for initial polixenic culture, and TYI-S-33 (Diamond's) medium for monoxenic and axenic cultures. In order to eliminate the microorganism contaminating the stools the following antibiotics were used penicillin, streptomicin, ampicillin, chloramphenicol, kanamycin, nistatin, ceftriaxone and amphoterycin B. Both starins grew in similar culture conditions with a yield of 2 x 10 elevation a 6 microorganism per tube of 15 ml. Both strains belog to pathogenic zymodemes, and virulence was determined by the capacity for producing hepatic abscesses in 100 percentage of the hamsters inoculated intrahepatically