Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
Mem. Inst. Oswaldo Cruz
; 89(3): 463-9, Jul.-Sept. 1994. ilus
Article
em En
| LILACS
| ID: lil-164121
Biblioteca responsável:
BR15.1
ABSTRACT
The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Reação em Cadeia da Polimerase
/
Leishmania
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
1994
Tipo de documento:
Article
/
Congress and conference