Diagnosis of malaria by acridine orange fluorescent microscopy in an endemic area of Venezuela
Mem. Inst. Oswaldo Cruz
;
91(1): 83-6, Jan.-Feb. 1996. tab
Artigo
em Inglês
| LILACS
| ID: lil-164140
ABSTRACT
Fluorescent (acridine orange) microscopical examination of capillary centrifuged blood (quantitative buffy coat [QBC] analysis) and Giemsa stained thick blood smears (GTS) were compared for diagnosis of malaria in blood specimens from adults living in malaria transmission areas of the states of Bolivar and Amazonas in southeastern and south Venezuela, respectively. Of a total of 198 GTS examined, 95 subjects (48 por cento) showed parasitaemia. Among the 95 blood films with a positive GTS, 94 were judged positive by the QBC. However, positive QBC tubes were found in 29 out of 103 blood specimens with a negative GTS. Thus, relative to a GTS standard, the sensitivity and specificity of the QBC-test was 99.2 por cento and 72 por cento, respectively. Young trophozoites of Plasmodium vivax and P. falciparum could not be distinguished with certainty. It is confirmed that the OBC offers many advantages compared with the standard diagnosis of malaria parasites, specifically in the speed of staining and ease of interpretation. However, in places where P. falciparum and P. vivax occur, species and stage differentation should be confirmed with the GTS.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Áreas de Pobreza
/
Malária
/
Microscopia de Fluorescência
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Venezuela
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
1996
Tipo de documento:
Artigo
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