Effect of plasma freezing temperature, anticoagulant and time of storage on factor VIII: C activity in cryoprecipitate

RESUMO

The influence of ACD and CPDA-1 anticoagulants, and storage time for 3 and 6 months on F VIIIC activity were compared in cryprecipitate obtained at -70ºC, and -30ºC plasma freezing temperature. To eliminate variations in F VIIIC activity between donor plasma, the cryoprecipitation at -70ºC and -30ºC was made in paired plasma volumes (approximately 100 ml) from each blood unit. Employing ACD plasmas (n= 50), there was no significant difference in F VIIIC activity between cryoprecipitate prepared at -70ºC (X=31.1 IU/bag) and -30ºC was made in paired plasma volumes (approximately 100 ml) from each blood unit. Employing ACD plasmas (n= 50), there was no significant difference in F VIIIC activity between cryprecipitate prepared at -70ºC (X= 31.1 IU/bag) and -30ºC (X = 30.5 IU/bag), and the storage did not modifify FVIIIC activity. In contrast, in cryprecipitate prepared from CPDA-1 plasma (n= 31), the F VIIIC levels obtained at-30ºC (X= 43.8IU/bag) were significantly higher than those at-70ºC (X=37.3 IU/bag), but a deterioration of F VIIIC activity (about 50 percent) was observed after 6 months of cryprecipitate storage. Therefore, if cryprecipitate is stored it stored it would be more convenient to use ACD instead of CPDA-1 and make cryoprecipitation either at-70ºC or-30ºC