Enzyme-linked thyroglobulin immunoassay
Rev. Hosp. Säo Paulo Esc. Paul. Med
;
6(3/4): 87-90, July-Dec. 1995. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-180111
ABSTRACT
A one-step enzyme linked sandwich immunoassay using Silicone rods coated with rabbit anti-human thyroglobulin anti-Tg) immunoglobulin G (Fab') conjugated with beta-D-galactosidase was established for the measurement of thyroglobulin in human sera. The volume of serum needed for the assay was 2 mul The sensitivity of the assay was 1.52 amol/tube, corresponding to O.5 ng/ml. The precision was proven by coefficients of variation intra-assay, 7.0 to 9.1 per cent inter-assay, 5.3 to 7.4 per cent. The correlation between this EIA and RIA was O.91, p < O.O1.
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Índice:
LILACS (Américas)
Assunto principal:
Autoanticorpos
/
Tireoglobulina
/
Imunoglobulina G
/
Beta-Galactosidase
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Rev. Hosp. Säo Paulo Esc. Paul. Med
Assunto da revista:
Medicina
Ano de publicação:
1995
Tipo de documento:
Artigo
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