Acetylcholinesterase release from crude mitochondrial fractions and superfused synaptosomes of rat hypothalamus / Liberatión de acetilcolinesterarasa de la fracción mitocondrial cruda y de sinaptosomas superfundidos de hipotalamo de rata

ABSTRACT

Crude mitochondrial fractions of rat hypothalamus, incubated in oxygenated Krebs solution (37ºC), released acetylcholinesterase (AChE) (increased of enzyme activity) into the medium, upon depolarization by 50 mM K+;this effect was absent in Ca²+ free medium. Superfused-synaptosomes obtained from subfractionated crude mitochondrial fractions of rat hypothalamus also released AChE, in a calcium-dependent fashion, when dependent fashion, when depolarized by 50 mM K+ or 50 µM veratridine. The veratridine-releasing effect was antagonized by 2 mM tetrodotoxin. Lactic dehydrogenase activity did not change in the medium with the introduction of depolarizing substances