Association between polyclonal B cell activation and the presence of autoantibodies in mice infected with Yersina enterocolitica O:3
Braz. j. med. biol. res
;
30(3): 401-5, Mar. 1997. graf
Artigo
em Inglês
| LILACS
| ID: lil-191352
RESUMO
Eight-week old conventional female Swiss mice were inoculated intravenously with Yersinia enterocolitica O3. A second group of normal mice was used as control. Five mice from eaeh group were bled by heart puncture and their spleens were removed for spleen cell collection on the 3rd, 5th, 7th, 1Oth, l4th and 21st day after infection. Immunoglobulin-secreting spleen cells were detected by the isotypespecific protein A plaque assay. Total immunoglobulin levels were determined in mouse serum by single radial immunodiffusion and the presence of autoantibodies was determined by ELISA. We observed a marked increase in the total number of cells secreting immunoglobulins of all isotypes as early as on the 3rd day post-infection and the peak of secretion occurred on the 7th day. At the peak of the immunoglobulin response, the total number of secreting cells was 19 times higher than that of control mice and most immunoglobulin-secreting cells were of the IgG2a isotype. On the 10th day post-infection, total serum immunoglobulin values were 2 times higher in infected animals when compared to the control group, and continued at this level up to the 2lst day post-infection. Serum absorption with viable Y. enterocolitica cells had little effect on antibody levels detected by single radial immunodiffusion. Analysis of serum autoantibody levels revealed that Y. enterocolitica infection induced an increase of antimyosin and anti-myelin immunoglobulins. The sera did not react with collagen. The present study demonstrates that Y. enterocolitica O3 infection induces polyclonal activation of murine B cells which is correlated with the activation of some autoreactive lymphocyte clones.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Autoanticorpos
/
Baço
/
Yersinia enterocolitica
/
Yersiniose
/
Linfócitos B
Tipo de estudo:
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1997
Tipo de documento:
Artigo
/
Congresso e conferência
/
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