The role of protein kinases in antigen-activation of peripheral blood mononuclear cells of Schistosoma mansoni infected individuals
Mem. Inst. Oswaldo Cruz
;
92(5): 613-7, Sept.-Oct. 1997. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-194203
RESUMO
T cell recognition of antigens displayed on the surface of antigen presenting cell results in rapid activation of protein tyrosine kinases and kinases C. This process leads to second messengers, such as inositol phosphates and diacylglycerol, and phosphorylation of multiple proteins. The role of different protein kinases in the activation of peripheral blood mononuclear cells (PBMC) from Schistosoma mansoni infected individuals was evaluated using genistein and H-7, specific inhibitors of protein tyrosine kinase and kinase C, respectively. Our results showed that proliferation in response to soluble egg antigen or adult worm antigen preparation of S. mansoni was reduced when PBMC were cultured in presence of protein kinase inhibitors. Using these inhibitors on in vitro granuloma reaction, we also observed a marked reduction of granuloma index. Taken together, our results suggest that S. mansoni antigen activation of PBMC involves protein kinases activity.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Proteínas Quinases
/
Esquistossomose
Limite:
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
1997
Tipo de documento:
Artigo
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