A 28-fold increase in secretory protein synthesis is associated with DNA puff activity in the salivary gland of Bradysia Hygida (Diptera, Sciaridae)
Braz. j. med. biol. res
;
30(5): 605-14, May 1997. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-196671
ABSTRACT
When the first group of DNA puffs is active in the salivary gland regions S1 and S3 of Bradysia hygida larvae, there is a large increase in the production and secretion of new salivary proteins demonstrable by [3H]-Leu incorporation. The present study shows that protein separation by SDS-PAGE and detection by fluorography demonstrated that polypeptides range in molecular mass from about 23 to 100 kDa. Furthermore, these proteins were synthesized mainly in the S1 and S3 salivary gland regions where the DNA puffs C7,C5,C4 and B10 are conspicuous, while in the S2 region protein synthesis was very low. Others have shown that the extent of amplification for DNA sequences that code for mRNA in the DNA puffs C4 and B10 was about 22 and 10 times, respectively. The present data for this group of DNA puffs are consistent with the proposition that gene amplification is necessary to provide some cells with additional gene copies for the production of massive amounts of proteins within a short period of time (Spradling AC and Mahowald AP (1980) Proccedings of the National Academy of Sciences, USA, 77 1096-1100).
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Peptídeos
/
Saliva
/
Glândulas Salivares
/
Técnicas In Vitro
/
Sondas de DNA
/
Amplificação de Genes
/
Dípteros
Tipo de estudo:
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1997
Tipo de documento:
Artigo
/
Documento de projeto
Similares
MEDLINE
...
LILACS
LIS