In situ hybridization and immunoperoxidase for quick detection and identification of leishmania braziliensis in tissue
Parasitol. día
;
20(3/4): 86-90, jul.-dic. 1996. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-202465
ABSTRACT
The combined used of in situ hybridization and immunoperoxidase techniques applied to histological sections of the biopsy allowed an increment of the efficiency in the detection of Leishmania diagnosis.The molecular basis for DNA diagnosis is to allow labelled single-stranded species or strain-specific DNA sequences, selected from well characterised reference species, to find and hybridize with homologous DNA from or in, the unknown isolates of parasites. The use of immunoperoxidase assay was useful in disclosing the antigen pathways and revealed amastigotes and antigenic material in various morphological patterns
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Índice:
LILACS (Américas)
Assunto principal:
Leishmania braziliensis
/
Técnicas Imunoenzimáticas
/
Hibridização In Situ
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Parasitol. día
Assunto da revista:
Parasitologia
Ano de publicação:
1996
Tipo de documento:
Artigo
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