Metabolic post-feeding changes in flat body and hemolymph of Dipetalogaster maximus (Hemiptera: Reduviidae)

RESUMO

Lipids and glycogen in fat body as well as the modifications in the wet weight of this organ were evaluated in an unfed insect, Dipetalogaster maximus, on day 5 after adult acdysis (time 0) and during a 30-day period after ingestion of blood meal. Total lipids, high density lipophorin (HDLp), carbohydrates, total protein and uric acid were determined in the hemolymph during the same period. Fat body wet weight was maximum on day 10 post-feeding and represented on day 30 only 42 per cent of the maximum weight. Lipids stored in the fat body increased up to day 15 reaching 24 per cent of the total weight of tissue. Glycogen was maximum on day 20, representing approximately 3 per cent of the fat body weight. HDLp represented at all times between 17-24 per cent of the total proteins, whose levels ranged between 35 and 47 mg/ml. Uric acid showed at 20, 25 and 30 days similar levels and significantly higher than the ones shown at days 10 an 15. Hemolymphatic lipids fluctuated during starvation between 3-4.4 mg/ml and carbohydrates showed a maximum on day 15 after a blood meal, decreasing up to 0.26 mg/ml on day 25. The above results suggest that during physiological events such as starvation, the availability of nutrients is affected, involving principally the fat body reserves.