Cultivo in vitro de condrocitos y reparo de superficies articulares en animales

RESUMO

The objetive of this experimental study is to achieve the production in vitro of inmature cartilaginous cells and its conservation, storing and effectiveness in the repairement of injured articular surfaces. After a broad bibliographic revision of the osteoarticular and chodral allografts as well as the recent studies of culture isolated cells of cartilage, we decited of carry out in a practical way and according to our enviroment along term prospective study of animal comparative experimental chararter with the initial aim to standarized a technique for the obtention of free articular cells as well as to achieve its conservation and obtain its reproduction in vitro using the F-10 HAM medium. After to create articular defaults in the knee of mature rabbits, we graffed with cells previously cultured in the laboratory, we present the preliminar hystologic, morphologic and biochemical results using the mentioned technic. If we obtain good results and an adecuate statistics tests, we will apply the same techinc in human at the label of minor articulations. With the first results we obtained a viability of 80 por cent (with coloration of Trypan-blue), formation of metachromatic reaction (with Toluidin-blue) and a satisfactory result on the 16 week of the allografts; it will be valorated by macroscopic scale of Salter-O'Driscoll; microscopic seale of Shigeyuki and the hystologic rebound criterion of kawabe. In addition we gain the celular crioconservation for more than 120 days using de DMSO 10 por cent as the crioprotector following the schemes and postulates of Schachar and Tomford; avoiding damage of the functions an viability, which was mantain in about 80 por cent