Semi-quantitative PCR: A tool to study low abundance messages in the kidney
Arch. med. res
;
28(1): 55-60, mar. 1997. ilus
Artigo
em Inglês
| LILACS
| ID: lil-225196
RESUMO
Some specific functions are often localized to unique cellular types or structures in organs such as kidney, brain, blood, and endocrine glands. As a result, it is not ucommon that gene producta, although heavily expressed in some cell type within these organs, ultimately appear as low abundance products when total RNA is probed, resulting in decreased power of the conventional Northern blot analysis. To study gene expression in these circumstances, more sensitive techniques like RNAse protection assay and quantitative or semi-quantitative PCR strategies have been developed. In the present study, we provede a detailed descriptio of the semiquantitative PCR strategy in our laboratory. Using specific primers to amplify fragments from the neuronal isoform of the nitric oxide synthase and the thiazide-sensitive Na+Cl- contransporter (low abundance messages in the kidney), we show that the semi-quantitative PCR strategy is a valuable tool when low abundance messages are to be studied
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Índice:
LILACS (Américas)
Assunto principal:
RNA Mensageiro
/
Proteínas de Transporte
/
Regulação da Expressão Gênica
/
Reação em Cadeia da Polimerase
/
Ratos Wistar
/
Rim
Limite:
Animais
Idioma:
Inglês
Revista:
Arch. med. res
Assunto da revista:
Medicina
Ano de publicação:
1997
Tipo de documento:
Artigo
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