Molecular analysis of snRNAs and scRNAs using autoantibodies from patients with systemic lupus erythematosus
Arch. med. res
;
28(4): 571-5, dec. 1997. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-225265
ABSTRACT
Immunoprecipitation analysis of total HeLa cells RNA extract byprotein A-Sheparose purified autoantibodies and pCp 32P-3' end labeling RNAs revealed that U1, U2, U4 and U5 snRNAs are related with anti-Sm or U1nRNP autoantibodies, while the hY1, hY3, hY4 and hY5 scRNAs were related to anti-SSA/Ro autoantibodies present in sera of patient with Systemic Lupus Erythematosus. The authors detected molecular snRNAs and scRNAs specificities by autoantibodies in 71 sera, the molecular RNA specificity for anti-Sm (U1, U2, U4 and U5 snRNAs) was present in 39 percent; anti-SSA/Ro sera reacted against scRNAs (hY1, hY3, hY4 and hY5) in 36 percent, then anti-U1nRNP sera recognized U1 snRNA in 13 percent of sera and anti-rRNP related with rRNA were recognized in 8 percent. Twenty-nine SLE sera were RNA negative. A molecular characterization of the autoantibodies in sera from SLE patients may be a useful tool for clinical and laboratory diagnosis of SLE, and the use of autoantibodies es molecular probes allows to continue exploring some basic mechanism of gene expression
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Índice:
LILACS (Américas)
Assunto principal:
Autoanticorpos
/
RNA
/
Testes de Precipitina
/
Células HeLa
/
RNA Nuclear Pequeno
/
Lúpus Eritematoso Sistêmico
Limite:
Humanos
Idioma:
Inglês
Revista:
Arch. med. res
Assunto da revista:
Medicina
Ano de publicação:
1997
Tipo de documento:
Artigo
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