Serodiagnosis of Trypanosoma cruzi infection using the new particle gel immunoassay - ID-PaGIA Chagas
Mem. Inst. Oswaldo Cruz
;
94(1): 77-82, Jan.-Feb. 1999. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-225934
RESUMO
The ID-Chagas test is a particle gel immunoassay (PaGIA). Red coloured particles are sensitised with three different synthtic peptides representing antigen sequences of Trypanosoma cruzi Ag2, TcD and TcE. When these particles are mixed with serum containing specific antibodies, they agglutinate. The reaction mixture is centrifuged through a gel filtration matrix allowing free agglutinated particles to remain trapped on the top or distributed within the gel. The result can be read visually. In order to investigate the ability of the ID-PaGIA to discriminate negative and positive sera, 111 negative and 119 positive, collected in four different Brazilian institutions, were tested by each of the participants. All sera were previously classified as positive or negative according to results obtained with three conventional tests (indirect immunofluorescence, indirect hemaglutination, and enzyme linked immunosorbent assay). Sensitivity rates of ID-PaGIA varied from 95.7 per cent to 97.4 per cent with mean sensitivity of 96.8 per cent and specificity rates varied from 93.8 to 98.8 per cent with mean specificity of 94.6 per cent. The overall Kappa test was 0.94. The assay presents as advantages the simplicity of operation and the reaction time of 20 min. In this study, ID-PaGIA showed to be highly sensitive and specific.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Trypanosoma cruzi
/
Testes Imunológicos
/
Doença de Chagas
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
1999
Tipo de documento:
Artigo
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